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Horizontal ultrathin‐layer multi‐zonal electrophoresis of DNA: An efficient tool for large‐scale polymerase chain reaction (PCR) fragment analysis
Author(s) -
van den Berg Bartel M.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181524
Subject(s) - silver stain , pipette , electrophoresis , polymerase chain reaction , chromatography , staining , chemistry , gel electrophoresis , dna , microbiology and biotechnology , layer (electronics) , analytical chemistry (journal) , biology , biochemistry , genetics , gene , organic chemistry
A rapid and efficient procedure for large‐scale analysis of polymerase chain reaction (PCR) fragments in the range of 200–3000 base pairs is presented. The procedure is based on horizontal ultrathin‐layer multi‐zonal (HUME) electrophoresis of PCR fragments in polyacrylamide gels followed by silver staining. HUME gels can be prepared rapidly using a simple procedure called the flap technique. The electrophoretic set‐up allows the use of multi‐channel pipettes for sample loading. Separation and detection of the PCR fragments from sample preparation to silver staining can be carried out in 2 h. Using four electrophoresis units, one technician can analyze 400 PCR fragment samples in 2 h.