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Unique identification of proteins from small genome organisms: Theoretical feasibility of high throughput proteome analysis
Author(s) -
Cavalcoli James D.,
VanBogelen Ruth A.,
Andrews Philip C.,
Moldover Brian
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181503
Subject(s) - proteome , identification (biology) , computational biology , genome , throughput , biology , proteomics , genetics , computer science , gene , botany , telecommunications , wireless
We evaluate current levels of accuracy for estimation of molecular weight ( M r ) and isoelectric point (p I ) to proteins on two‐dimensional (2‐D) gels as well as the distribution and clustering of proteins in the predicted proteome of E. coli . We also examine the ability to find single candidates within the predicted proteome for matching to a protein seen on 2‐D gels, based on the current level of accuracy. We discuss the levels of accuracy needed to match predicted proteins to observed proteins based solely on M r and p I criteria obtained from genomic information, and propose methodology to achieve this level of accuracy. In addition, we will address the future goals of this work since the small genomes of bacteria provide a foundation and stepping stone to similar studies in higher organisms.