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Developments in amino acid analysis using capillary electrophoresis
Author(s) -
Smith Joel T.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181228
Subject(s) - capillary electrophoresis , derivatization , amino acid , enantiomer , chromatography , amino acid analysis , chemistry , resolution (logic) , moiety , biochemistry , organic chemistry , high performance liquid chromatography , computer science , artificial intelligence
The current status in the analysis of amino acids using capillary electrophoresis is addressed. This area of biological analysis has received increased attention with more than 200 articles being published in the last five years. This review discusses pre‐, post‐, and on‐column derivatization techniques used to tag amino acids providing a detectable moiety. Several separation methodologies which provided resolution for large sets of amino acids are presented. An overview of advances in the enantiomeric resolution methodologies for amino acids is given. Both direct and indirect enantiomeric separation schemes are summarized. Recent advances in detection strategies for both derivatized and underivatized amino acids are presented. Applications utilizing amino acid analysis by capillary electrophoresis are described. This review covers articles published between 1991 and 1996.