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Capillary electrophoresis of biotechnology‐derived proteins
Author(s) -
Strege Mark A.,
Lagu Avinash L.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181225
Subject(s) - capillary electrophoresis , isoelectric focusing , chromatography , micellar electrokinetic chromatography , chemistry , electrophoresis , sodium dodecyl sulfate , gel electrophoresis , free flow electrophoresis , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , biochemistry , enzyme
Applications of capillary electrophoretic techniques for the analysis of biotechnology‐derived proteins continue at a steady pace. This review summarizes the analyses of these proteins achieved using capillary isoelectric focusing, micellar electrokinetic capillary chromatography, capillary electrophoresis/mass spectrometry and capillary gel electrophoresis. A very brief summary of each technique is included. Application of capillary isoelectric focusing to the analysis of recombinant tissue plasminogen activator in quality control type applications is examined in some detail. Analyses of dosage forms of biotechnology derived proteins of pharmaceutical importance as well as the identification and examination of the peptides, obtained after enzymatic cleavage of proteins, by capillary electrophoresis have been included. Examples of capillary gel electrophoresis as a substitute sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) of biotechnology‐derived proteins are also reviewed.