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Polymeric separation media for capillary electrophoresis of nucleic acids
Author(s) -
Sunada Wade M.,
Blanch Harvey W.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181215
Subject(s) - nucleic acid , polymer , capillary electrophoresis , matrix (chemical analysis) , chemistry , electrophoresis , nucleic acid quantitation , chromatography , organic chemistry , biochemistry
The choice of polymer matrix for separating nucleic acids by capillary electro‐phoresis has often been arbitrary. However, considerable research in the area has led to a wealth of data exploring the key parameters of the polymer matrix that affect nucleic acid separations: polymer type, polymer molecular mass, polymer concentration, temperature, and buffer components and additives. Using this information, it is possible to use rational methods of choosing a good polymer matrix for a particular application. Further research into the properties of the mechanism of separation in polymer solutions, as well as the polymer matrix and other solution components will lead to even more efficient separations.