Premium
Capillary electrophoresis in presence of sodium dodecyl sulfate and a sieving medium
Author(s) -
Takagi Toshio
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181214
Subject(s) - sodium dodecyl sulfate , capillary electrophoresis , chemistry , polymer , chromatography , electrophoresis , gel electrophoresis , sodium , molecular mass , biochemistry , organic chemistry , enzyme
For analysis of proteins in presence of sodium dodecyl sulfate (SDS), basic knowledge about their behavior in presence and absence of sieving medium is required. Capillary electrophoresis (CE) is particularly suitable for this purpose. Although various polymers are used as sieves in CE analysis in presence of SDS, attention must be paid to possible interaction between SDS and the polymer. For obtaining new insights polymers with defined molecular weight and distribution should be utilized.