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Pre‐, on‐ and post‐column derivatization in capillary electrophoresis
Author(s) -
Bardelmeijer Heleen A.,
Waterval Joop C. M.,
Lingeman Henk,
Hof Ron van 't,
Bult Auke,
Underberg Willy J. M.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181212
Subject(s) - derivatization , capillary electrophoresis , chromatography , reagent , chemistry , analyte , aldehyde , high performance liquid chromatography , organic chemistry , catalysis
This survey gives a short overview of the various reagents and procedures that can be used for pre‐, post‐ and on‐column derivatization in capillary electrophoresis. First there is an introduction about capillary electrophoresis as an analytical technique; this is followed by a discussion of the pros and cons of the various modes of derivatization and a comparison with liquid chromatography. In the following paragraphs the reagents for a number of functional groups are discussed. The emphasis is on derivatization of the amino group. Most of the information on the reagents and derivatization procedures is listed in tables together with information on the detection mode, analytes, sensitivity and samples. In addition to the amino group, information is given on labeling of aldehyde, keto, carboxyl, hydroxyl and sulfhydryl groups.