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Integrated chip‐based capillary electrophoresis
Author(s) -
Effenhauser Carlo S.,
Bruin Gerard J. M.,
Paulus Aran
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181211
Subject(s) - capillary electrophoresis , chromatography , microfluidics , multiplex , capillary action , derivatization , chip , micellar electrokinetic chromatography , capillary electrochromatography , chemistry , nanotechnology , materials science , computer science , high performance liquid chromatography , bioinformatics , telecommunications , composite material , biology
Abstract Integrated capillary electrophoresis (ICE) is emerging as a new analytical tool allowing fast, automated, miniaturized and multiplexed assays, thus meeting the needs of the pharmaceutical industry in drug development. The current state‐of‐the‐art of ICE is described with an emphasis on the choice of the support material (glass or polymeric materials), electrokinetic fluid handling, and injection and detection issues. Strategies and chip designs for pre‐ or post‐column derivatization, DNA sequencing, on‐line PCR analysis, on‐chip enzymatic sample digestion, fraction isolation, and immunoassays are presented. The review concludes with a brief outlook.