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Methods for the estimation of binding constants by capillary electrophoresis
Author(s) -
Rundlett Kimber L.,
Armstrong Daniel W.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181210
Subject(s) - capillary electrophoresis , electrophoresis , chemistry , constant (computer programming) , association (psychology) , chromatography , computer science , programming language , philosophy , epistemology
Capillary electrophoresis (CE) has developed into a particularly effective means to determine apparent equilibrium constants for molecular association in solution ( e.g. , to micelles, cyclodextrins, antibiotics, proteins, RNA, DNA. etc. ). The various experimental, graphical and mathematical approaches for determining association constants are reviewed. In CE, association constants can be calculated because there is a relationship between substrate concentration and the measured electrophoretic mobility of the solute. Most of the approaches for obtaining association constants by CE are conceptually and mathematically related to one another. Likewise, they are analogous to many spectroscopic techniques that are used for obtaining association constants. The advantages, limitations and proper use of the various CE approaches are examined.