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Affinity capillary electrophoresis
Author(s) -
Rippel Geza,
Corstjens Hugo,
Billiet Hugo A. H.,
Frank Johannes
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181208
Subject(s) - capillary electrophoresis , affinity electrophoresis , chemistry , ligand (biochemistry) , electrophoresis , feature (linguistics) , chromatography , combinatorial chemistry , affinity chromatography , biochemistry , enzyme , receptor , philosophy , linguistics
The application of affinity capillary electrophoresis (ACE) to the study of molecular interactions is reviewed. ACE appears to be a sensitive, versatile and convenient tool to obtain reliable data on binding constants and stoichiometries of interacting systems using the Hummel‐Dreyer method and variants thereof. A powerful feature is the possibility to analyze simultaneously the affinity of a large number of compounds for the same ligand, making it a promising tool for the screening of large combinatorial libraries.