Premium
Peak broadening in capillary zone electrophoresis
Author(s) -
Gaš Bohuslav,
Štědrý Milan,
Kenndler Ernst
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181203
Subject(s) - laminar flow , joule heating , dispersion (optics) , capillary electrophoresis , capillary action , mechanics , electromigration , diffusion , flow (mathematics) , electric field , chemistry , electro osmosis , aperture (computer memory) , electrophoresis , materials science , analytical chemistry (journal) , optics , thermodynamics , physics , chromatography , quantum mechanics , composite material , acoustics
Abstract A review on peak broadening in capillary zone electrophoresis in free solutions is given which covers a selection of the literature published on this topic over the period mainly between 1992 and the beginning of 1997 (consisting of 71 publications). The contributions to peak dispersion from extracolumn effects ( e.g. due to the finite length of the injection zone, or the aperture of the detector), from longitudinal diffusion, Joule heating, electromigration dispersion (concentration overload), a different path length of the solute ions, wall adsorption, laminar flow and the (longitudinally) homogeneous or nonhomogeneous electroosmotic flow are described. The latter may also occur when a longitudinally nonhomogeneous radial electric field is applied. Peak dispersion is depicted either by the plate‐height model, or the concentration of the solute as a function of space and time is calculated either analytically or numerically by solving the equation of continuity with appropriate initial and boundary conditions and possibly completed by equations governing further quantities.