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Common nuclear matrix proteins in rat tissues
Author(s) -
Korosec Thomas,
Gerner Christopher,
Sauermann Georg
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181138
Subject(s) - nuclear matrix , matrix (chemical analysis) , nuclear protein , interphase , proteome , nucleus , chemistry , cell nucleus , biology , microbiology and biotechnology , biochemistry , dna , transcription factor , gene , chromatography , chromatin
Nuclear matrix proteins have been defined as insoluble residual proteins resulting from treatment of isolated nuclei with nucleases, detergents and high ionic strength buffers. They are considered as in part representing the proteins constituting the three‐dimensional framework of the interphase nucleus. Though cell‐specific nuclear matrix proteins have been differentiated from ubiquitously occurring (common) nuclear matrix proteins, the number and types of common nuclear matrix proteins have not yet been unequivocally established. In the present study nuclear matrix proteins were prepared from isolated nuclei of rat kidney, liver, lung, spleen and testes. The matrix proteins were separated by two‐dimensional (2‐D) electrophoresis and silver stained. Then the spot patterns were compared by computer‐assisted image analysis. Composite images were derived for nuclear matrix proteins of individual tissues. Finding between 396–483 spots per tissue, a total of 964 individual spots were registered. Of these, 102 were common nuclear matrix proteins, as appearing in each of the tissue‐characteristic images. The apparent molecular mass and p I data may serve for further identification of these nuclear proteins.