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Simple and rapid determination of the acetaldehyde dehydrogenase (ALDH2) genotypes by nonradioactive single‐strand conformation polymorphism analysis
Author(s) -
Yuasa Isao,
Umetsu Kazuo,
Nakagawa Mayumi,
Ikebuchi Jun,
Inoue Terutaka,
Irizawa Yoshito
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181109
Subject(s) - genotyping , aldh2 , single strand conformation polymorphism , genotype , biology , genetics , aldehyde dehydrogenase , microbiology and biotechnology , alcohol dehydrogenase , acetaldehyde , polymorphism (computer science) , enzyme , biochemistry , polymerase chain reaction , gene , ethanol
The genotyping of mitochondrial acetaldehyde dehydrogenase (ALDH2) is very important in alcohol studies. We describe an ALDH2 genotyping method based on nonradioactive single‐strand conformation polymorphism (SSCP) analysis on mini‐gels following amplification with a mutated primer set. The three ALDH2 genotypes were clearly and unambiguously distinguished. This method was applied to the ALDH2 genotyping of 129 unrelated Japanese. The allele frequency of ALDH2*2 was calculated to be 0.271, which was consistent with the previous data. The method proved to be simple, rapid and reliable, and dispensed with isotopic reagent and expensive restriction enzymes and equipment. The SSCP method described here is valuable in routine ALDH2 genotyping.