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Reorientation of large DNA molecules in concentrated polyacrylamide solution during crossed‐field electrophoresis
Author(s) -
Oana Hidehiro,
Doi Masao,
Ueda Masanori,
Yoshikawa Kenichi
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181104
Subject(s) - electrophoresis , polyacrylamide , dna , gel electrophoresis of nucleic acids , pulsed field gel electrophoresis , electric field , gel electrophoresis , polyacrylamide gel electrophoresis , chemistry , molecule , field (mathematics) , chromatography , biophysics , analytical chemistry (journal) , biology , biochemistry , physics , polymer chemistry , mathematics , organic chemistry , quantum mechanics , genotype , pure mathematics , gene , enzyme
Recently, we found that, in concentrated neutral solutions, DNA molecules migrate in linear conformation under steady electric field. In this paper, we report the conformational change of DNA during 120° crossed‐field electrophoresis in the same polymer solution. We found that, in concentrated polyacrylamide solutions, the reorientation process of DNAs becomes simple: the DNA goes back along the previous track and the reorientation time is longer motion for the separation of DNA fragments in pulsed field gel electrophoresis. We expect that this phenomenon is useful for a more efficient separation technique of large DNAs than the current pulsed field gel electrophoresis.