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Clinical application of capillary electrophoresis to unconcentrated human urine proteins
Author(s) -
Jenkins Margaret A.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181020
Subject(s) - urine , bence jones protein , chromatography , chemistry , capillary electrophoresis , electropherogram , tamm–horsfall protein , proteinuria , electrophoresis , capillary action , antibody , biochemistry , kidney , biology , immunology , immunoglobulin light chain , materials science , endocrinology , composite material
Urine protein electrophoresis can be performed by capillary electrophoresis using spun unconcentrated urine diluted with running buffer. The separation which utilises the excellent sensitivity of capillary electrophoresis gives electropherograms similar to those obtained by diluting concentrated urine with buffer. A 72 cm × 50 μm internal diameter (ID) fused silica capillary was used for the analysis which took less than 15 min. Bence Jones protein can be detected from unconcentrated urine over urine protein concentrations ranging from 9.7 g/L to 0.04 g/L. Other clinical patterns, such as glomerular protein‐uria, the presence of intact immunoglobulin and Tamm Horsfall protein can also be detected. The benefit of using unconcentrated urine is the time and cost saved by not concentrating the urine.