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Development of a nonisotopic capillary electrophoresis‐based method for measuring glomerular filtration rate
Author(s) -
Bergert Jan H.,
Liedtke Robert R.,
Oda Robert P.,
Landers James P.,
Wilson David M.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181018
Subject(s) - renal function , urine , chromatography , capillary electrophoresis , chemistry , urology , filtration (mathematics) , coefficient of variation , kidney , capillary action , medicine , materials science , biochemistry , statistics , mathematics , composite material
The conditions for quantitative measurement of nonisotopic iothalamate meglumine (Conray) in urine and plasma by capillary zone electrophoresis (CE) have been developed. The impetus for developing this methodology was to replace the traditional [ 125 I]iothalamate glomerular filtration rate (GFR) marker assay, a routine tool in the measurement of kidney function. This new approach for measuring kidney function is attractive since it avoids the cost of administration of radioisotopic compounds to patients, as well as the cost associated with purchase and disposal of isotopic compounds and contaminated samples. The concentration of iothalamate in urine and plasma determined by CE can be used directly to calculate GFR. The GFR in patients injected with [ 125 I]iothalamate and nonisotopic iothalamate simultaneously showed an excellent correlation (0.998) with between‐day coefficient of variation of 2.30% and a recovery of 102% and 98%, respectively, when added to urine and plasma. Interference from drugs and other urinary compounds is eliminated with this method. Collectively, this study has shown that CE is a cost‐effective alternative to the current methodology for measuring GFR.