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Identification of monoclonal proteins in serum: A quantitative comparison of acetate, agarose gel, and capillary electrophoresis
Author(s) -
Katzmann Jerry A.,
Clark Raynell,
Wiegert Elaine,
Sanders Elisabeth,
Oda Robert P.,
Kyle Robert A.,
NamystGoldberg Colette,
Landers James P.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181011
Subject(s) - chromatography , capillary electrophoresis , agarose , chemistry , agarose gel electrophoresis , electrophoresis , monoclonal antibody , identification (biology) , monoclonal , biochemistry , biology , dna , antibody , immunology , botany
A selected group of 308 sera were analyzed by capillary electrophoresis (CE), agarose gel electrophoresis (AGE), and cellulose acetate electrophoresis (CAE) and evaluated for abnormalities that would suggest the presence of a monoclonal protein. The sensitivity (an electrophoretic abnormality in sera that contained a monoclonal protein) and specificity (a normal electrophoretic pattern in sera that did not contain a monoclonal protein) was determined for each electrophoretic procedure. CAE was the most specific procedure and CE was the most sensitive. The increase in sensitivity of CE was primarily due to increased detection of cryoglobulins and free light chains. The quantitation of the gamma region and/or monoclonal antibody peaks by CE was similar to results obtained by AGE. Quantitation of very large monoclonal protein peaks (> 3.0 g/dL) by on‐line absorption detection (CE) yielded higher results than quantitation by dye‐binding (AGE).