Premium
Microsatellite‐based cancer detection using capillary array electrophoresis and energy‐transfer fluorescent primers
Author(s) -
Wang Yiwen,
Hung SuChun,
Linn Jürgen F.,
Steiner Gabriel,
Glazer Alexander N.,
Sidransky David,
Mathies Richard A.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181007
Subject(s) - microsatellite , capillary electrophoresis , fluorescence , chromatography , tandem , chemistry , microbiology and biotechnology , allele , biology , materials science , genetics , optics , gene , physics , composite material
The development of sensitive, rapid, and accurate methods and apparatus for high‐throughput short tandem repeat (STR) analysis will be critical for the use of microsatellite alteration in cancer screening. Here we show that STR‐based bladder cancer diagnosis can be performed using capillary array electrophoresis and two‐color labeling with energy‐transfer (ET) fluorescent primers. Rapid (≤35 min) separations are achieved on capillary arrays using replaceable separation matrices and the allelic ratios are quantitatively determined with a precision of ± 10%. With this precision, a variation of 20% was considered diagnostically significant. These methods provide a significant improvement in the speed, ease, and precision of STR analyses compared to slab gel electrophoresis.