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Informative enzyme/probe combinations for the multilocus DNA fingerprinting of marsupials
Author(s) -
Lambert Kim,
Jones Michelle,
Shimmin Glenn,
Sofronidis George,
Bowden Don,
Taggart David,
TempleSmith Peter
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180937
Subject(s) - marsupial , tammar wallaby , biology , zoology , population , restriction enzyme , oligomer restriction , dna profiling , brushtail possum , evolutionary biology , microsatellite , genetics , dna , oligonucleotide , gene , allele , demography , sociology
DNA fingerprinting has become an invaluable tool in the study of population genetics, paternity success, and individual identification; however, the species specificity of some methods has made the wide‐range screening of many different species very time‐consuming. In this study we describe the development and application of reliable and informative DNA fingerprinting techniques in a range of marsupial species using three different restriction enzyme and two oligonucleotide probe combinations. Six species from four marsupial families, the koala (Phascolarctidae), tammar wallaby (Macropodidae), southern hairynosed wombat (Vombatidae), kowari, and dusky and brown marsupial mice (Dasyuridae) were examined. Restriction enzymes Hin fI Alu I and Hae III were used in combination with the digoxygenin (DIG)‐labelled oligonucleotide probes (CAC) 5 and (GGAT) 4 . The combinations of Hin fI/(GGAT) 4 , Alu I/(CAC) 5 and Alu I/(GGAT) 4 were the most informative, providing highly resolved bands, low background, and the lowest band sharing between individuals. The genetic diversity evident within the different species showed a clear relationship between the level of band sharing and population size. The greatest levels of band sharing were found in the kowaris (80%), which were part of a long‐term captive colony originating from a few founders, and the lowest levels of band sharing were found in the marsupial mice (30–35%) and tammar wallaby (45%), which were caught from large outbred wild populations.