Premium
Escherichia coli proteome analysis using the gene‐protein database
Author(s) -
VanBogelen Ruth A.,
Abshire Kelly Z.,
Moldover Brian,
Olson Eric R.,
Neidhardt Frederick C.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180805
Subject(s) - proteome , open reading frame , escherichia coli , biology , gene , database , gel electrophoresis , computational biology , proteomics , polyacrylamide gel electrophoresis , two dimensional gel electrophoresis , microbiology and biotechnology , genetics , biochemistry , peptide sequence , computer science , enzyme
The gene‐protein database of Escherichia coli is a collection of data, largely generated from the separation of complex mixtures of cellular proteins on two‐dimensional (2‐D) polyacrylamide gel electrophoresis. The database currently contains about 1600 protein spots. The data are comprised of both identification information for many of these proteins and data on how the level or synthesis rates of proteins vary under different growth conditions. Three projects are underway to further elucidate the E. coli proteome including a project to localize on 2‐D gels all of the open reading framed encoded by the E. coli chromosome, a project to determine the condition(s) under which each open reading frame is expressed and a project to determine the abundance and location of each protein in the cell. Applications for proteome databases for cell modeling are discussed and examples of applications in therapeutic drug discovery are given.