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Isoelectric properties of the soluble matrices in relation to the chemical composition of some Scleractinian skeletons
Author(s) -
Dauphin Yannicke,
Cuif JeanPierre
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180726
Subject(s) - chemical composition , composition (language) , isoelectric focusing , chemistry , isoelectric point , chromatography , biochemistry , organic chemistry , philosophy , enzyme , linguistics
Soluble matrices of four Scleractinian skeletons ( Madrepora, Favia, Leptastrea and Fungia ), were extracted and studied by isoelectric focusing (IEF). The main part of the extracted matrices is acidic. IEF gels are poorly stained or unstained by Coomassie Blue and silver. The positive Alcian Blue staining is indicative of a sulfated and acidic nature of the glycoproteins. Chemical analysis shows high S contents in Scleractinian skeletons. These results are supported by the comparison with IEF and chemical contents of Molluscan shells.