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Separation of disaccharides as their borate complexes by capillary electrophoresis with indirect detection in visible range
Author(s) -
Plocek Jiří,
Chmelík Josef
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180721
Subject(s) - capillary electrophoresis , chromophore , boron , chromatography , chemistry , electrophoresis , electrolyte , analytical chemistry (journal) , capillary action , photochemistry , materials science , organic chemistry , electrode , composite material
Borate complexation was used to make possible the separation of disaccharides by capillary electrophoresis with indirect detection. A high borate concentration did not affect the indirect detection sensitivity in as negative a way as predicted previously. The concentration sensitivity for sucrose was determined to be 2 m M at the borate concentration of 200 m M in running electrolyte. The newly introduced backround chromophore, p ‐nitrophenol, allows the monitoring of the separation process in a visible range at 400 nm. This also enables the indirect detection of UV‐absorbing compounds in complex mixtures in which they would be impossible to detect with a UV‐absorbing backround chromophore.