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Identification of wallaby milk whey proteins separated by two‐dimensional electrophoresis, using amino acid analysis and sequence tagging
Author(s) -
Molloy Mark P.,
Herbert Ben R.,
Yan Jun X.,
Williams Keith L.,
Gooley Andrew A.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180708
Subject(s) - identification (biology) , amino acid , chromatography , electrophoresis , bovine milk , biology , peptide sequence , biochemistry , chemistry , whey protein , botany , gene
Micropreparative two‐dimensional polyacrylamide gel electrophoresis has been used to separate milk whey proteins from the Tammar wallaby ( Macropus eugenii ). We have used a combination of amino acid analysis and N ‐terminal sequence tagging as a rapid and sensitive method to identify the major whey proteins. Using these techniques, we confidently identified α‐lactalbumin and late lactation protein. While these are the only two M. eugenii whey proteins with a corresponding SWISS‐PROT entry, we demonstrate that by using amino acid analysis and matching across species boundaries, we can identify previously unsequenced conserved wallaby whey proteins including β‐lactoglobulin and serum albumin.