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Enantiomer separation of denopamine by capillary electrophoresis with charged and uncharged cyclodextrins
Author(s) -
Ishibuchi Kiyotaka,
Izumoto Shinichi,
Nishi Hiroyuki,
Sato Tadashi
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180624
Subject(s) - capillary electrophoresis , enantiomer , chemistry , capillary action , chromatography , cyclodextrin , amine gas treating , electrophoresis , capillary electrochromatography , organic chemistry , materials science , composite material
Direct separation of enantiomers of denopamine was investigated by capillary electrophoresis employing charged and uncharged cyclodextrin (CD) derivatives. Uncharged β‐type CDs, having hydrophobic groups, were essential for the enantioseparation of denopamine; of these, especially dimethyl‐β‐CD was effective. Among charged CDs, γ‐type as well as β‐type CDs were found effective for the enantioseparation of denopamine. Reversal of migration order of R ‐form (active) and S ‐form enantiomers was investigated by using two types of coated capillaries: (i) an amine capillary with an inner wall coated with dimethylamino groups, and (ii) a polyacrylamide‐coated capillary. Manipulation of migration order could be easily performed by selecting suitable capillaries, buffer pH, and CDs.