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Chiral interactions in capillary zone electrophoresis: Computer simulation and comparison with experiment
Author(s) -
Ingelse Benno A.,
Sarmini Karim,
Reijenga Jetse C.,
Kenndler Ernst,
Everaerts Frans M.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180614
Subject(s) - capillary electrophoresis , electropherogram , mandelic acid , cyclodextrin , analyte , chemistry , chromatography , electrophoresis , chiral derivatizing agent , enantiomer , capillary action , sensitivity (control systems) , analytical chemistry (journal) , materials science , stereochemistry , organic chemistry , chiral column chromatography , high performance liquid chromatography , electronic engineering , engineering , composite material
Chiral interaction in capillary electrophoresis can be modeled using p K values, mobilities of analytes, and their formation constants with the chiral selector. An existing steady‐state simulation program for CE (HPCESIM) was recently extended with a chiral submenu involving the chiral parameters listed above. These were experimentally determined in both our laboratories for mandelic acid and terbutaline using hydroxypropylated β‐cyclodextrin as chiral selector. A comparison was made between both sets of parameters and between experimental electropherograms and those obtained from simulation. Error analysis of the results indicate the sensitivity of the obtained results.