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Central composite design in the chiral analysis of amphetamines by capillary electrophoresis
Author(s) -
Varesio Emmanuel,
Gauvrit JeanYves,
Longeray Rémi,
Lantéri Pierre,
Veuthey JeanLuc
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180613
Subject(s) - capillary electrophoresis , central composite design , robustness (evolution) , chromatography , enantiomer , biological system , linear regression , quadratic equation , response surface methodology , robustness testing , capillary action , composite number , design of experiments , resolution (logic) , chemistry , regression analysis , analytical chemistry (journal) , materials science , computer science , mathematics , algorithm , statistics , composite material , artificial intelligence , stereochemistry , biochemistry , geometry , biology , gene , fuzzy logic
We have studied a central composite design for the chiral separation of amphetamines using capillary electrophoresis. Five variables, i.e. , buffer concentration, pH, chiral selector concentration, temperature, and applied voltage, were investigated. Enantiomeric resolutions as well as analysis time and generated power were established as responses for each experiment. A model of each response was obtained by multiple regression of a quadratic‐degree mathematical expression. From the models, we determined the most favorable conditions for the chiral separation by optimizing the resolution and setting the other responses at threshold values. Results were compared with a previous study in which a systematic investigation of the operating parameters was carried out. In order to visualize the robustness of the method, response surfaces were drawn for the significant variables. We have concluded that experimental designs offer a rapid means of optimizing several variables and provide an efficient test for the robustness of the analytical method.