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Determination of association constants of dansyl‐amino acids and β‐cyclodextrin in N ‐methylformamide by capillary electrophoresis
Author(s) -
Valkó István E.,
Sirén Heli,
Riekkola MarjaLiisa
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180611
Subject(s) - capillary electrophoresis , chemistry , electrolyte , cyclodextrin , chromatography , electrophoresis , solubility , electrode
The use of nonaqueous background electrolytes in capillary electrophoresis (CE) is a promising new trend which should widen the scope of this technique. We demonstrate the chiral separation of dansyl‐amino acids (Dns‐AAs) in N ‐methylformamide (NMF) using β‐cyclodextrin (β‐CD) as chiral selector. The solubility of β‐CD is much better in NMF than in water, allowing high concentration of the chiral selector and successful enantioseparation despite the weak host‐guest interaction between the Dns‐AAs and β‐CD. The association constants for the complexation between Dns‐AAs and β‐CD could be calculated from the electrophoretic mobilities, with attention paid to the change in viscosity of the electrolyte upon addition of β‐CD. The association constants ranged between 2 and 13 M −1 .