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Three approaches to enantiomer separation of β‐adrenergic antagonists by capillary electrochromatography
Author(s) -
Nilsson Staffan,
Schweitz Leif,
Petersson Maria
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180606
Subject(s) - capillary electrochromatography , enantiomer , pindolol , chemistry , alprenolol , cyclodextrin , electrochromatography , chromatography , atenolol , molecularly imprinted polymer , propranolol , capillary electrophoresis , organic chemistry , selectivity , receptor , medicine , biochemistry , radiology , blood pressure , catalysis
Abstract Three different capillary electrochromatographic methods for the enantiomer separation of β‐adrenergic antagonists (acebutolol, alprenolol, atenolol, metoprolol, pindolol, prenalterol, and propranolol) were applied using different cyclodextrins (β‐cyclodextrin, carboxymethyl‐β‐cyclodextrin and dimethyl‐β‐cyclodextrin) added to the electrolyte, a cross‐linked protein‐gel (cellobiohydrolase I) and a molecularly imprinted (( R )‐enantiomer of propranolol) superporous polymer as chiral selectors. Through use of these different separation strategies, all the β‐adrenergic antagonists studied could be resolved into their enantiomers, although the three methods were carried out without extensive optimization. The protein and molecularly imprinted phases gave the highest selectivities whereas employing cyclodextrins resulted in the highest separation efficiency. Proteins and cyclodextrins are primarily natural products, albeit the cyclodextrins can be derivatized. In contrast, the molecularly imprinted chiral stationary phase can be highly customized when produced.