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Analysis of proteins from different phase variants of the entomopathogenic bacteria Photorhabdus luminescens by two‐dimensional zymography
Author(s) -
Ong Kevin L.,
Chang Frank N.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180530
Subject(s) - photorhabdus luminescens , zymography , biology , proteases , protease , microbiology and biotechnology , biochemistry , photorhabdus , enzyme , gene
Two‐dimensional zymography which combines two‐dimensional electrophoresis with zymography was used to analyze proteases and other proteins produced by different phase variants of two strains of Photorhabdus luminescens . Both the primary and secondary phases of P. luminescens strains Hp and Hm secreted proteases. The protease in P. luminescens Hp has a molecular weight ( M r ) of 57 000 and an isoelectric point (p I ) of 4.4 whereas that in P. luminescens Hm has an M r of 59 000 and p I of 4.9. Several putative protease degradation products were clearly visible in the zymograms from both bacterial strains. Two‐dimensional zymography also showed that several secretory proteins were present only in particular phase variants and therefore could be used as specific markers. Unexpectedly, the two‐dimensional zymography revealed that a nonsecretory protease with an M r of 47 000 and a p I of 4.0 was present in the cell extracts of all phases of both P. luminescens Hp and Hm. The application of the two‐dimensional zymography for the identification of other enzymes was also discussed.