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Prothymosin α: Efficient sequence determination by experimental and theoretical capillary electrophoretic support
Author(s) -
Weiler Angelika,
Stoeva Stanka,
Tsitsilonis Ourania E.,
Voelter Wolfgang
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180516
Subject(s) - capillary electrophoresis , sequence (biology) , electrophoresis , chromatography , chemistry , capillary action , computational biology , biological system , biology , biochemistry , physics , thermodynamics
A rapid and efficient primary structure elucidation of prothymosin (ProTα) and its enzymatic fragments was performed by a combination of matrix‐assisted laser desorption ionization mass spectrometry (MALDI), automatic N ‐terminal Edman degradation, and the available theoretical predictions of electrophoretic mobility in capillary electrophoresis (CE) as a basis suggesting active sites of ProTα in the different bioassays Presented at the “Elektrophorese Forum ”96” meeting of the German Electrophoresis Society, held at the Technical University Munich, October 23–25; 1996 .