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The different expression of proteins recognized by monoclonal anti‐heat shock protein 70 (hsp70) antibody in human colonic diseases
Author(s) -
Stul Jiřri,
Bureš Jan,
Jandík Pavel,
Langr František,
Kovářová Hana,
Macela Aleš
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180347
Subject(s) - hsp70 , heat shock protein , monoclonal antibody , immunostaining , gene isoform , pathological , biology , pathology , immunohistochemistry , microbiology and biotechnology , hsp60 , ulcerative colitis , antibody , disease , immunology , medicine , biochemistry , gene
Analysis of the expression of hsp70 (70 kDa heat‐shock proteins) in normal and pathological tissues might prove their potential diagnostic and prognostic values. In the present study, we combined high‐resolution two‐dimensional polyacrylamide gel electrophoresis with immunoblotting to study hsp70 expression in normal, preneoplastic and neoplastic colonic mucosa. By monoclonal anti‐hsp70 antibody, recognizing both the constitutive and inducible forms of hsp70, we have detected six charge isoforms localized in the pH 5.1–5.3 range and in the molecular mass range of 68–69 kDa in normal colonic mucosa. Immunostaining of hsp70 in polypous and malignant tissues revealed qualitative as well as quantitative changes in the expression of more acidic isoforms of hsp70 in comparison with normal tissue. Furthermore, the different basic isoforms of hsp70 were detected in chronically inflamed colonic mucosa from patients suffering from ulcerative colitis (UC) or Crohn's disease (CD). Besides the standard hsp70 protein pattern, additional proteins with molecular masses of about 39, 40, 74 and 75 kDa were variably immunostained in normal and pathological specimens. These observations suggest that hsp70 expression may be closely linked to disease etiology and/or pathophysiology.