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Large‐scale protein modelling and integration with the SWISS‐PROT and SWISS‐2DPAGE databases: The example of Escherichia coli
Author(s) -
Peitsch Manuel C.,
Wilkins Marc R.,
Tonella Luisa,
Sanchez JeanCharles,
Appel Ron D.,
Hochstrasser Denis F.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180326
Subject(s) - database , computer science , protein structure database , software , web server , scale (ratio) , data mining , world wide web , biology , programming language , the internet , sequence database , genetics , physics , quantum mechanics , gene
Knowledge-based molecular modelling of proteins has proven useful in many instances, including the rational design of mutagenesis experiments, but it has generally been limited by the availability of expensive computer hardware and software. To overcome these limitations, we developed the SWISS-MODEL server for automated knowledge-based protein modelling. The SWISS-MODEL server uses the Brookhaven Protein Data Bank as a source of structural information and automatically generates protein models for sequences which share significant similarities with at least one protein of known three-dimensional structure. We have now used the software framework of the server to generate large collections of protein models, and established the SWISS-MODEL Repository, a new database for automatically generated and theoretical protein models. This repository is directly integrated with the SWISS-PROT and SWISS-2DPAGE databases through the ExPASy World Wide Web server (URL is http://expasy.hcuge.ch). Here we present an illustration of this process by an application to the Escherichia coli sequences.