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Analysis of toxic drugs by capillary electrophoresis using polyacrylamide‐coated columns
Author(s) -
Jinno Kiyokatsu,
Han Yinghong,
Sawada Hirokazu
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180217
Subject(s) - polyacrylamide , capillary electrophoresis , chemistry , chromatography , sodium dodecyl sulfate , copolymer , isopropyl , acrylamide , buffer (optical fiber) , micelle , electrophoresis , isopropyl alcohol , aqueous solution , polymer chemistry , organic chemistry , polymer , telecommunications , computer science
Toxic drugs, including barbiturates and benzodiazepines, were analyzed using polyacrylamide‐coated columns in capillary electrophoresis (CE). The separations were carried out in absence of electroosmotic flow. Seven kinds of barbiturates were successfully separated with the coated columns in free solution without further additives. Benzodiazepines, the electrically neutral solutes, were introduced onto the coated column, and separated in presence of sodium dodecyl sulfate above its critical micelle concentration in the running buffer. This CE method offered fast and efficient separation of more hydrophobic solutes, such as benzodiazepines. The separation of seven barbiturates was studied in linear (noncross‐linked) polyacrylamide solutions and in acrylamide / N ‐isopropylacrylamide (AA/IPAA) copolymer solutions to explore the effect of isopropyl groups in the AA/IPAA copolymer chain.