Premium
The effect of cyclodextrin modifiers on electrophoretic separation of aromatic hydrocarbons
Author(s) -
Nguyen AnLac,
Luong John H. T.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180212
Subject(s) - chemistry , benzene , cyclodextrin , toluene , capillary electrophoresis , xylene , chromatography , ether , diphenyl ether , electrophoresis , ethylbenzene , organic chemistry
Cyclodextrin‐modified capillary electrophoresis has been developed for separation and analysis of benzene and its derivatives. The procedure used a mixture of negatively charged sulfobutyl ether‐β‐cyclodextrin (SBβCD) and neutral hydroxypropyl‐β‐cyclodextrin (HPβCD) to effect differential distribution (partitioning) of the aromatic hydrocarbons between the buffer and CD phases. In 80 m M phosphate buffer, containing 15 m M SBβCD and 5 m M HPβCD, benzene, toluene, ethyl benzene and three xylene isomers (BTEXs) were well resolved with a number of theoretical plates well above 100 000, for 50 cm of effective length. Some halogenated benzenes were also observed to separate well from the BTEX components to indicate their suitability as an internal standard for BTEX analyses. Equilibrium complexation models were used for investigating the effect of the cyclodextrin(s) at different concentrations and temperature on the electrophoretic mobility.