Premium
Potential of two‐dimensional electrophoresis in routine identification of closely related durum wheat lines
Author(s) -
Picard Philippe,
BourgoinGrenèche Mireille,
Zivy Michel
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180131
Subject(s) - mahalanobis distance , linear discriminant analysis , identification (biology) , pattern recognition (psychology) , spots , biological system , euclidean distance , biology , artificial intelligence , chromatography , computational biology , computer science , chemistry , botany
Four closely related curum wheat varieties were compared by computer‐assisted analysis of two‐dimensional electrophoretic maps of leaf proteins. A low inter‐varietal polymorphism was revealed and seven reliable qualitatively varying proteins allowed rapid visual identification of genotypes. For numerous spots, presence/absence or quantitative variations were greatly affected by a batch effect. Several criteria that should be used to discard unreliable spots or gels a priori were reviewed. Nevertheless, it was shown that, provided that the experimental design allows the integration of the batch effect, screening for discriminant markers as well as computing distances based on protein quantity variations are possible and allow variety identification. Euclidean and Mahalanobis distances allowed variety discrimination and single gel classification with a minimum risk of error, not only by taking into account the quantitative variations in discriminant proteins selected by analysis of variance, but also by taking into account all reproducible spots. The possible applications of two‐dimensional electrophoresis in variety identification are discussed.