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Translationally controlled tumor protein: A protein identified in several nontumoral cells including erythrocytes
Author(s) -
Sanchez JeanCharles,
Schaller Dominique,
Ravier Florence,
Golaz Olivier,
Jaccoud Sylviane,
Belet Monique,
Wilkins Marc R.,
James Richard,
Deshusses Jacques,
Hochstrasser Denis
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180127
Subject(s) - monoclonal antibody , gene isoform , biology , cell culture , microbiology and biotechnology , antibody , cytoplasm , biochemistry , gene , immunology , genetics
The translationally controlled tumor protein (TCTP) is a growth‐related protein which is regulated at the translational level. It is present in mammals, higher plants and Saccharomyces cerevisiae . This study was undertaken to localize and further characterize the TCTP in human cell lysates using two‐dimensional gel electrophoresis, monoclonal antibodies, and 45 Ca‐gel overlay. TCTP was found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It could not be detected in kidney and renal cell carcinoma (RCC). A monoclonal antibody raised against TCTP detected three isoforms likely due to post‐translational modifications. A calcium binding property was found as well as heat stability and cytoplasmic localization. The high degree of homology from plants to man and its expression in many tissues suggests that TCTP most likely has a cell housekeeping function.