Premium
Deoxyribonuclease I‐facilitated electrotransfer of protein‐DNA complexes from electrophoretic gels to nitrocellulose membranes
Author(s) -
Ivanchenko Maria,
Zlatanova Jordanka
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180115
Subject(s) - nitrocellulose , deoxyribonuclease , electrophoresis , membrane , dna , chemistry , biochemistry , chromatography , microbiology and biotechnology , biology
A simple and reproducible technique for efficient transfer of protein‐DNA complexes from electrophoretic gels to nitrocellulose membranes is described. Transfer of DNA‐protein complexes may be difficult, especially when the DNA is of high molecular mass. A considerable improvement in the efficiency of transfer can be achieved by directly digesting the DNA in the gel by DNase I. The method is illustrated in the example of the preferential binding of histone H1 to superhelical plasmid DNA.