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Resolution of high molecular weight proteins in dependence on electric field strength in polyacrylamide gel electrophoresis
Author(s) -
StaritaGeribaldi Mireille,
Houri André
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180112
Subject(s) - electrophoresis , electric field , resolution (logic) , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , gel electrophoresis , polyacrylamide , pulsed field gel electrophoresis , high resolution , chemistry , chromatography , analytical chemistry (journal) , gel electrophoresis of nucleic acids , biochemistry , physics , enzyme , computer science , remote sensing , quantum mechanics , artificial intelligence , genotype , polymer chemistry , gene , geology
Resolution of high molecular weight proteins, in the upper region of polyacrylamide gels, was studied in relation to the type of electric field. Separations by constant field gel electrophoresis (CFGE) were compared to those in pulsed oscillatory high‐performance electrophoresis (POPE), a novel technique which allows electrophoresis at high field strengths owing to a novel local field distribution. This distribution contributes to structural and mechanical stability of the gel with resultant well‐reproducible separation, enhanced resolution, and higher absolute mobility of proteins in POPE.