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Detection of conformation bias between the complementary strands of deoxynucleotide repeat (TG/AC) n
Author(s) -
Dobi Albert,
Mahan Mark A.,
Agoston Denes V.
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150180104
Subject(s) - antiparallel (mathematics) , dinucleotide repeat , chemistry , acrylamide , electrophoresis , crystallography , biophysics , polymer , biochemistry , biology , physics , allele frequency , organic chemistry , quantum mechanics , magnetic field , genotype , copolymer , gene
Abstract Antiparallel complementary strands of DNA contribute symmetrically to the helical structure and its stability; rough conformational differences between complementary strands would interfere with this symmetric contribution and the stability. To detect conformational differences between complementary strands, we challenged the complementary strands to different “pore size” of acrylamide gels and measured differences in migration during electrophoretic mobility analysis. Changes in migration differences revealed a significant structural bias between the complementary strands of the dinucleotide repeat deoxy‐(TG/AC) n .