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Separation of aracytidine and cytidine by capillary electrophoretic techniques
Author(s) -
Křivánková Ludmila,
Košťálová Andrea,
Vargas Gabriela,
Havel Josef,
Boček Petr
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150171225
Subject(s) - capillary electrophoresis , chromatography , cytidine , detection limit , chemistry , analyte , cytarabine , absorbance , sodium dodecyl sulfate , electrophoresis , cytidine deaminase , biochemistry , leukemia , enzyme , medicine
Aracytidine (cytarabine, 1‐β‐D‐arabinofuranosylcytosine) is a synthetic analog of cytidine in which ribose is substituted by arabinose; it is used as a drug for the treatment of leukemia. A fast and reliable capillary electrophoretic method for the analysis of cytarabine and cytidine is described. The procedure utilizes the interactions with sodium dodecyl sulfate (SDS) micelles and borate, present in the background electrolyte, for the mobilization and selective separation of the analytes. The detection is carried out by UV absorbance at 275 nm. The method was applied both to pharmaceutical preparations and human serum. Analysis of an untreated serum requires 15 min; the detection limit is 0.8 μg/mL and the relative standard deviation (RSD) is 5.3%.