Premium
Use of wide‐bore capillaries in constant denaturant capillary electrophoresis
Author(s) -
Li ZiaoCheng,
Thilly William G.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150171213
Subject(s) - capillary electrophoresis , capillary action , chromatography , chemistry , electrophoresis , joule heating , dna , analytical chemistry (journal) , materials science , biochemistry , composite material
A serious limitation of capillary electrophoresis in separating mutant from wild‐type DNA sequences is the amount of DNA which may be loaded on narrow‐bore columns. Because we sought to examine mutant sequences derived from more than 10 9 human cells, we were obliged to overcome this limitation. Thus we examined the feasibility of using wide‐bore capilllaries of 250, 320 and 540 μm in constant denaturant capillary electrophoresis (CDCE) using conditions which successfully limited the effect of Joule heating. Excellent separations were observed using the wide‐bore capillaries comparable to those obtained using a 75 μm capillary. DNA loading capacities were increased almost 100‐fold as the bore of capillary increased from 75 to 540 μm.