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Probing the microheterogeneity of O‐specific chains from Yersinia ruckeri using capillary zone electrophoresis/electrospray mass spectrometry
Author(s) -
Bateman Kevin P.,
Banoub Joseph H.,
Thibault Pierre
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150171205
Subject(s) - capillary electrophoresis , chemistry , chromatography , mass spectrometry , glycan , capillary electrophoresis–mass spectrometry , tandem mass spectrometry , electrospray , hydrolysis , electrophoresis , yersinia ruckeri , electrospray ionization , biochemistry , biology , fish <actinopterygii> , rainbow trout , glycoprotein , fishery
The analysis of underivatized oligosaccharides arising from mild acid hydrolysis of endotoxins from Yersinia ruckeri serotype 02 was achieved using on‐line capillary zone electrophoresis‐electrospray mass spectrometry (CZE‐ESMS). This technique provided unparalleled resolution of the differnt glycans obtained from purified fractions of the native endotoxins or from hydrolysis of lipopolysaccharides from Y. ruckeri. Electrophoretic conditions enabling the separation of anionic and cationic analytes were developed to determine possible sites of heterogeneity on either the core or the O‐chain glycans. Structural characterization of underivatized oligosaccharides identified in the ion electropherograms was achieved using tandem mass spectrometry under lowcollision energy conditions NRCC 39726. .