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Rapid loading of large sample volumes, analyte cleanup, and modified moving boundary transient isotachophoresis conditions for membrane preconcentration‐capillary electrophoresis in small diameter capillaries
Author(s) -
Tomlinson Andy J.,
Benson Linda M.,
Jameson Stephen,
Naylor Stephen
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150171203
Subject(s) - isotachophoresis , capillary electrophoresis , chromatography , analyte , capillary action , chemistry , mass spectrometry , electrophoresis , analytical chemistry (journal) , tandem mass spectrometry , membrane , materials science , electrolyte , biochemistry , electrode , composite material
Using a removable membrane preconcentration (mPC) cartridge, large sample volumes can be loaded prior to final assembly of the mPC capillary electrophoresis (CE) capillary. For narrow‐bore (⩽ 25 μm ID) uncoated mPC‐CE capillaries, applied to peptide analysis, efficient moving boundary transient isotachphoresis (tITP) conditions at the onset of electrophoresis are described. The enhancement of mPC‐CE‐mass spectrometry (MS) technology afforded by rapid sample loading and modified moving boundary tITP conditions are demonstrated by analysis of major histocompatibility complex (MHC) class I peptides that were derived from a K b precipitation of mouse EL‐4 cells. Furthermore, we demonstrate the structural characterization of these immunologically significant molecules by mPC‐CE‐tandem mass spectrometry (mPC‐CE‐MS/MS).