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Random amplified polymorphic DNA (RAPD) interpretation requires a sensitive method for the detection of amplified DNA
Author(s) -
Valentini Alessio,
Timperio Anna Maria,
Cappuccio Irene,
Zolla Lello
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150171009
Subject(s) - rapd , primer (cosmetics) , agarose gel electrophoresis , dna , capillary electrophoresis , microbiology and biotechnology , biology , polymerase chain reaction , gel electrophoresis , genetics , agarose , electrophoresis , chromatography , chemistry , gene , population , demography , organic chemistry , sociology , genetic diversity
The random amplified polymorphic DNA technique (RAPD) has found wide use in molecular genetics because of its speed and ease of use. For various reasons, with this method the amplified DNA fragments are produced at different concentrations between genotypes and even between polymerase chain reaction (PCR) runs. Since the detection of the multiple amplified fragments is performed routinely by agarose gel, and seldom by acrylamide gel electrophoresis, we have found that by capillary zone electrophoresis (CZE), which is more sensitive and accurate than gel electrophoresis, it is possible to unequivocally detect amplified fragments even at low concentration, avoiding polymorphism misinterpretation. CZE is also useful to make more potentially polymorphic fragments evident per random primer used, with obvious economical benefits.