Premium
Validation of the applied biosystems Prism™ 377 automated sequencer for forensic short tandem repeat analysis
Author(s) -
Frazier Rachael R. E.,
Millican Emma S.,
Watson Stephanie K.,
Oldroyd Nicola J.,
Sparkes Rebecca L.,
Taylor Karen M.,
Panchal Sasikala,
Bark Linda,
Kimpton Colin P.,
Gill Peter D.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150171008
Subject(s) - multiplex , str analysis , tandem , analytical chemistry (journal) , dna sequencer , microsatellite , chromatography , computer science , chemistry , dna , materials science , genetics , dna sequencing , biology , biochemistry , composite material , gene , allele
The Applied Biosystems (ABI) Prism TM 377 DNA sequencer has been evaluated in an attempt to increase the throughput of samples for short tandem repeat (STR) analysis, in both forensic casework and the UK National Criminal Intelligence DNA Database. The gel system assessed consisted of 0.2 mm, 4% acrylamide 6 M urea gels, with a well‐to‐read distance of 36 cm. Gels were run at a constant voltage of 3 kV and constant temperature of 51°C. The run time of our second generation multiplex (SGM) STR system was achieved in less than 2 h. Rigorous validation has been performed on the instrument hardware and software Complete resolution of 1 base difference was obtained, up to and beyond 350 bases; sizing precision across gels was more than 2‐fold higher than the 373A and the sensitivity was increased by one third.