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Capture in the gel: Intermoleculare triplex formation during gel electrophoresis
Author(s) -
Belotserkovskii Boris P.,
Johnston Brian H.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150171004
Subject(s) - gel electrophoresis , electrophoretic mobility shift assay , electrophoresis , duplex (building) , gel electrophoresis of nucleic acids , two dimensional gel electrophoresis , dna , intermolecular force , chemistry , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , sequence (biology) , biophysics , chromatography , biology , biochemistry , gene , molecule , proteomics , gene expression , organic chemistry , enzyme
Analysis of unusual gel mobility patterns formed by certain DNA triplexes has revealed that intermolecular triplex formation can occur during gel electrophoresis when a faster migrating single strand overtakes a slower migrating band containing a duplex of appropriate sequence. Control experiments showed that this capture of the third strand occurs by sequence‐specific hybridization rather than some nonspecific retardation. This phenomenon can be used to detect triplexes by a gel‐shift assay even if their lifetime is much shorter than the time of gel electrophoresis.