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Activation energy of the separation of DNA sequencing fragments in denaturing noncross‐linked polyacrylamide by capillary elecrophoresis
Author(s) -
Fang Yu,
Zhang Jian Zhong,
Hou Joan Y.,
Lu Hui,
Dovichi Norman J.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170907
Subject(s) - reptation , polyacrylamide , arrhenius equation , activation energy , dna , capillary electrophoresis , capillary action , chemistry , polymer , analytical chemistry (journal) , chromatography , polymer chemistry , thermodynamics , physics , biochemistry , organic chemistry
We present the mobility of DNA sequencing fragments as a function of temperature in 5%T, 0%C noncross‐linked polyacrylamide and at an electric field of 150 V/cm. The mobility of the fragments follows the Arrhenius equation. The activation enegry for migration of a fragment through the polymer decreases monotonically with fragment length. The data were also analyzed in terms of the biased reptation model; the onset of biased reption with stretching occurs for longer fragments as the temperature increases. These results are quite different from those observed for separation of DNA in cross‐linked gels and represent a fundamental difference in the physics of the cross‐linked and noncross‐linked polyacrylamide.