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Computer optimization of background electrolyte composition in the separation of metal ions by capillary electrophoresis
Author(s) -
Billiet Hugo A. H.,
Andersson Per E.,
Haddad Paul R.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170815
Subject(s) - capillary electrophoresis , electropherogram , electrolyte , chemistry , chromatography , electrophoresis , metal ions in aqueous solution , metal , resolution (logic) , analytical chemistry (journal) , response surface methodology , ion , high performance liquid chromatography , electrode , computer science , organic chemistry , artificial intelligence
The iterative regression design developed earlier for high performance liquid chromatography (HPLC) has been applied successfully to the optimization of the separation of 11 metal ions by capillary electrophoresis. The parameters used for optimization were pH (over the range 3.5–5.0) and the concentration of 2‐hydroxyisobutyric acid (HIBA) (2–20 mM) used as the complex‐forming ligand added to the background electrolyte. Five initial experiments were performed and the response surface was calculated using the relative resolution product as criterion. Three additional experiments were sufficient to establish a stable optimum, with which all 11 metal ions were separated and well spread in the electropherogram. The total analysis time was less than 5 min.