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Interpretation of electrophoretic band shapes by a partition chromatographic model
Author(s) -
Weiss George H.,
Sokoloff Helen,
Zakharov Sergey F.,
Chrambach Andreas
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170809
Subject(s) - asymmetry , electrophoresis , agarose , gaussian , chemistry , partition (number theory) , mathematics , physics , statistical physics , chromatography , quantum mechanics , computational chemistry , combinatorics
Measurements of the shape of electrophoretic bands of phycoerythrin and conalbumin have been made at regular intervals during migration in agarose gels. Analysis of the peak shapes suggests the existence of a significant degree of asymmetry. This is to be contrasted with the symmetry around the peak associated with the generally assumed Gaussian band. The degree of asymmetry of the bands decreased as a function of time and increased with agarose concentration. A similar experiment on DNA indicated constancy of the degree of asymmetry as a function of time. These results can be interpreted as, but do not prove the validity of, a nonlocal diffusion equation which generalizes a theory originally put forth by Giddings and Eyring ( J. Am. Chem. Soc. 1955, 59 , 416–420). The results may be significant in framing a measure of the resolvability of electrophoretic peaks.