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Comparison of different electrophoretic methods for digital typing of the MS32 (D1S8) locus
Author(s) -
RodriguezCalvo María Sol,
Bellas Susana,
Soto Jose Luis,
Barros Francisco,
Carracedo Angel
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170803
Subject(s) - minisatellite , locus (genetics) , typing , polymerase chain reaction , silver stain , microbiology and biotechnology , biology , dna , population , agarose , allele , staining , genetics , microsatellite , gene , demography , sociology
Minisatellite variant repeat mapping by polymerase chain reaction (MVR‐PCR) is a new and powerful approach for individual identification from human DNA. This method has been successfully applied to the hypervariable human minisatellite MS32 (locus D1S8). In this work, we further investigate this locus using different electrophoretic techniques which include: agarose gels and nonradioactive hybridization, miniaturized polyacrylamide gels, followed by silver staining, and automatic detection of fluorescent labeled alleles. The advantages and disadvantages of each method are described. Also, MVR codes from a Galician population (NW Spain) are provided.